FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24347890
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG597400.023.48664580-81
AAGGGGG631650.022.31913682-83
TAGGGGG132550.018.8838750-51
TAAGGGG142350.017.83406350-51
AAAAAAG909300.016.99189878-79
GGGAAAA716700.016.55087722-23
GGGGAAA773550.016.43049422-23
AAAGGGG599500.016.38464580-81
GGGGGGA900400.015.90133720-21
GGGGGAA815750.015.79306920-21
GGGTTTT336550.015.58032442-43
AACGGGT32350.014.97565938-39
TTGGGGG475550.014.57699550-51
GGGAATA140650.014.43631964-65
CGGGTTT81900.014.15029740-41
TTAAGGG141600.014.0374848-49
GGTTTTT396500.014.021242-43
TTTTGGG385700.013.8535148-49
ACGGGTT44000.013.81710140-41
AAAAAGG748550.013.53110278-79
Produced by FastQC (version 0.11.5)