FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25704623
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG562350.023.20583380-81
AAGGGGG589000.022.25349482-83
TAGGGGG127400.018.06292250-51
GGGAAAA704600.016.82167622-23
GGGGAAA748600.016.36590822-23
TAAGGGG130850.016.35252250-51
AAAAAAG891500.015.96951978-79
GGGGGAA786800.015.939507520-21
GGGGGGA845450.015.729820-21
TTTTGGG443550.015.55381148-49
AAAGGGG555850.015.5432380-81
TTGGGGG489550.015.43122250-51
GGTTTTT408300.014.8782542-43
GGGAATA148400.013.98628364-65
TTAAGGG140700.013.72238748-49
AGGGGGG702650.012.84054382-83
AAAAAGG718700.012.42738278-79
AAAGTTT325550.012.28419428-29
TTTAGGG166000.012.18890648-49
GGAAAAA795800.012.16027524-25
Produced by FastQC (version 0.11.5)