FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23341786
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG561350.020.25678480-81
TTTTGGG411550.018.56340448-49
TAGGGGG130200.018.00260450-51
AGGGGGG799250.017.02104882-83
AAAAAGG685450.016.83477278-79
TTGGGGG547850.016.57621650-51
AAGGGGG593900.016.31596482-83
AAAAGGG562500.016.28038280-81
GGGGGGA982550.015.40571720-21
GGTTTTT336400.015.01538542-43
GGGGAAA592500.014.92609822-23
GGGGGAA710550.013.76643920-21
TTTAGGG138600.013.31333848-49
GGGAAAA520950.012.91899722-23
GGGAATA132800.012.80374764-65
TTTTTTG609050.012.35264546-47
TAAGGGG107050.011.55793450-51
GTTTTTT583950.011.3584842-43
TTAGGGG132350.011.250524548-49
AAAAAAG894800.011.23992978-79
Produced by FastQC (version 0.11.5)