FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32305320
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG848200.025.19927280-81
AAGGGGG906800.023.55596782-83
TAAGGGG200500.022.19677450-51
TAGGGGG175700.019.97732550-51
TTAAGGG200050.019.39753348-49
AAAAAAG1294000.018.03161678-79
GGGAAAA1082950.017.9858622-23
TTTTGGG570850.017.6973648-49
GGGGAAA1142300.017.34242822-23
TTGGGGG641200.016.8001950-51
GGGGGAA1212000.016.61180120-21
AAAGGGG834100.016.42863380-81
GGGGGGA1317850.016.04700320-21
GGGAATA200000.014.94939864-65
TTTAGGG198650.014.62079148-49
TTTAAGG270750.014.08672648-49
GAAAAAG648250.013.74119324-25
TTTGGGG622600.013.67841650-51
AAAGTTT453200.013.5139328-29
AAAAGTT458650.013.37412126-27
Produced by FastQC (version 0.11.5)