FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21450852
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG482350.021.5408680-81
AAGGGGG503550.020.9366982-83
TAGGGGG115850.018.2442150-51
GGTTTTT348350.018.16121542-43
AAAGGGG470150.017.17977380-81
GGGGAAA618800.015.9071622-23
GGGGGGA733700.015.76587320-21
TTTTGGG342000.015.63075548-49
GGGAAAA566600.015.59977122-23
TTGGGGG403800.015.43222650-51
TAAGGGG115950.015.3201150-51
GGGGGAA661200.015.23907520-21
AAAAAAG763050.014.97331578-79
GGGAATA122650.014.6378364-65
AGGGGGG621150.013.8298582-83
AAAAAGG610400.013.64058578-79
TTAAGGG120800.012.71943748-49
GGGGAAT163450.012.37883262-63
GGGGTTT230300.012.15758640-41
AGGGTTT201700.012.04473440-41
Produced by FastQC (version 0.11.5)