FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22096029
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG423200.017.8342580-81
AAGGGGG435850.017.06669882-83
AAAAGGG427800.016.75425180-81
AGGGGGG563650.015.20695282-83
GGGGGGA662650.015.14848120-21
GGGGTTT279150.014.453773540-41
GGGGGTT263450.014.33258140-41
AAAAAGG547450.014.10278478-79
GGGGAAA498150.013.90570222-23
TAGGGGG105750.013.81104750-51
GGTTTTT296450.013.68223442-43
GGGGGAA544400.013.37442420-21
GGGAAAA440600.012.63367722-23
TTTTGGG326200.012.34729448-49
TTGGGGG444550.012.09449450-51
GGGTTTT269300.011.80768642-43
GGGAATA113050.011.5535864-65
AAAAAAG710500.011.13044578-79
GGGGGGT403450.010.45988938-39
GGGGAAT150050.010.17662862-63
Produced by FastQC (version 0.11.5)