FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29718399
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1495050.5030721877043242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT738660.24855309332107697TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT343880.11571282827180564TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC528050.071.8774268
TCGGAAG575850.065.3880163
CGGAAGA582100.065.125044
ATCGGAA594700.063.798012
GATCGGA590100.062.769161
GAGCACA748100.051.14369
AAGAGCA788700.048.977347
GAAGAGC829600.045.9528856
GGAAGAG877150.043.775925
TATCTCG126850.040.60328740-41
CGTCTGA463050.040.4443216-17
CACGTCT468750.040.04905314-15
ACGTCTG465800.040.03801314-15
ACACGTC472150.039.9517112-13
TCTCGTA208250.039.66116342-43
CACACGT480550.039.42603712-13
CTCGTAT208200.038.48533644-45
GCACACG490000.038.47606710-11
GAACTCC480100.038.17784520-21
TGAACTC490150.038.14962820-21
Produced by FastQC (version 0.11.5)