FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22549993
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT7366623.2667948056569243TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1185550.063.512959
AGAGCAC1188900.063.2736478
CGGAAGA1226250.061.2506564
TCGGAAG1225350.061.237353
ATCGGAA1238600.060.5251352
GATCGGA1260750.059.3380931
AAGAGCA1283700.058.6752137
GAAGAGC1348350.055.91226
GGAAGAG1495650.050.5105825
TCGTATG814650.045.85640344-45
CGTATGC818200.045.80977246-47
TATGCCG818000.045.7680548-49
CTCGTAT810450.045.56358744-45
TCTCGTA811900.044.80177742-43
ATCTCGT820700.044.30112542-43
GTCACGA860250.043.57929228-29
ATGCCGT858150.043.49386648-49
GCCGTCT858450.043.4193650-51
ACGAGAT868800.043.3064632-33
CGAGATT866200.043.2417432-33
Produced by FastQC (version 0.11.5)