FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29775462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT7176832.4103169247214367TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1277000.058.502619
AGAGCAC1281950.058.2578938
TCGGAAG1318050.056.438373
CGGAAGA1321150.056.431794
ATCGGAA1331050.055.858792
GATCGGA1355850.054.662161
AAGAGCA1400800.053.4035537
GAAGAGC1478600.050.608536
TCGTATG807150.045.9271144-45
CGTATGC813650.045.6461846-47
TATGCCG813550.045.60139548-49
CTCGTAT808800.045.3635444-45
GGAAGAG1684450.044.6217965
TCTCGTA813750.044.51037242-43
ATCTCGT828500.043.70360642-43
GTCACGA859950.043.20887828-29
CGAGATT865350.042.95282432-33
ATGCCGT868350.042.66066448-49
ACGAGAT874200.042.63211432-33
AGTCACG874800.042.58672728-29
Produced by FastQC (version 0.11.5)