FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22431052
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6452672.8766684683357693TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1081750.061.8259778
GAGCACA1082850.061.8203289
TCGGAAG1104900.060.4154853
CGGAAGA1111500.060.125134
ATCGGAA1111250.060.0021252
GATCGGA1129700.058.8711971
AAGAGCA1169100.057.247757
GAAGAGC1241400.053.9175836
GGAAGAG1395700.048.117195
CGTATGC725250.045.8940346-47
TCGTATG723000.045.86448344-45
TATGCCG728900.045.60515248-49
CTCGTAT721550.045.41350644-45
TCTCGTA723950.044.64093442-43
ATCTCGT734750.044.06234742-43
GTCACGA765250.043.4122828-29
CGAGATT768950.043.22511332-33
ATGCCGT766150.043.22044448-49
AGTCACG776800.042.9961528-29
ACGAGAT777450.042.9480832-33
Produced by FastQC (version 0.11.5)