FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23084585
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT6408852.7762465732002544TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1047400.061.9403238
GAGCACA1050850.061.7868239
TCGGAAG1064500.060.7872243
ATCGGAA1068850.060.517872
CGGAAGA1075750.060.20014
GATCGGA1085600.059.3088231
AAGAGCA1143150.056.8234677
GAAGAGC1201550.054.1105356
GGAAGAG1359700.047.9185455
TCGTATG698200.045.9319844-45
CGTATGC702350.045.8382246-47
TATGCCG706300.045.5718348-49
CTCGTAT697100.045.4661144-45
TCTCGTA704800.044.38392342-43
ATCTCGT713400.043.86552442-43
GTCACGA743650.043.37536228-29
ATGCCGT746650.043.03590448-49
CGAGATT750450.042.92870332-33
GCCGTCT747800.042.8667450-51
AGTCACG754400.042.85173828-29
Produced by FastQC (version 0.11.5)