FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29086818
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT7613082.6173643332178855TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1319050.058.3481459
AGAGCAC1331750.057.7699288
CGGAAGA1396100.055.0550964
TCGGAAG1393850.055.0349043
ATCGGAA1413100.054.228042
AAGAGCA1451700.053.0884327
GATCGGA1447600.052.808121
GAAGAGC1532700.050.2940526
TCGTATG836700.045.5801544-45
CGTATGC840000.045.49283246-47
TATGCCG844350.045.26350448-49
CTCGTAT841700.045.11749644-45
TATCTCG847550.044.6022340-41
GGAAGAG1738700.044.510185
TCTCGTA853900.044.29295342-43
ATCTCGT868400.043.64090342-43
ATGCCGT892450.042.7574248-49
GCCGTCT896500.042.517550-51
GTATGCC900900.042.4149246-47
ACCGCTC901950.042.3603332-33
Produced by FastQC (version 0.11.5)