FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22528898
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6276692.7860617061695603TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1063950.058.96779
AGAGCAC1064250.058.8794148
CGGAAGA1115400.056.2026944
TCGGAAG1113550.056.1936843
ATCGGAA1127300.055.4871982
GATCGGA1150000.054.322071
AAGAGCA1156950.054.207257
GAAGAGC1226150.051.26616
GGAAGAG1374400.045.9160045
TCGTATG682850.045.61559344-45
CGTATGC683550.045.60941346-47
TATGCCG684950.045.4710648-49
CTCGTAT685650.045.16256744-45
TATCTCG690650.044.75087740-41
TCTCGTA695350.044.46517642-43
ATCTCGT706950.043.76917342-43
ATGCCGT723350.043.06044848-49
GCCGTCT726350.042.78596550-51
GTATGCC733050.042.5619946-47
CCGTCTT735250.042.34417352-53
Produced by FastQC (version 0.11.5)