FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26818620
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6018282.244067740994876TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1100950.056.0149739
AGAGCAC1101300.055.837088
TCGGAAG1150750.053.290543
CGGAAGA1159350.052.9321144
ATCGGAA1167650.052.462772
GATCGGA1192100.051.191591
AAGAGCA1215150.050.6413277
GAAGAGC1306700.047.203086
TCGTATG666550.045.58384744-45
CGTATGC673000.045.215246-47
TATGCCG675650.045.01618648-49
CTCGTAT674800.044.81887444-45
TATCTCG683000.044.17404640-41
TCTCGTA688950.043.81672342-43
ATCTCGT703450.042.98782742-43
ATGCCGT722650.042.0423948-49
GCCGTCT727000.041.75317850-51
GGAAGAG1482650.041.710615
GTATGCC733100.041.5246346-47
ACCGCTC737200.041.31128732-33
Produced by FastQC (version 0.11.5)