FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24986778
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT12729445.09447036348584TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2164700.058.9047289
AGAGCAC2204150.057.82828
AAGAGCA2335250.054.6228647
CGGAAGA2415150.052.7365724
TCGGAAG2443700.052.058143
GAAGAGC2455200.051.995866
ATCGGAA2504050.050.763952
GATCGGA2573450.049.2880971
GGAAGAG2703000.047.3336455
CGTATGC1373700.046.1255146-47
TATGCCG1374050.046.0633148-49
TCGTATG1372150.046.05048444-45
CTCGTAT1372450.045.83966444-45
TATCTCG1381700.045.3255540-41
TCTCGTA1386700.045.16549742-43
ATCTCGT1402550.044.77194642-43
ATGCCGT1412500.044.71356248-49
GTATGCC1424950.044.3465446-47
GCCGTCT1423050.044.2797550-51
CCGTCTT1432600.044.11517352-53
Produced by FastQC (version 0.11.5)