FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24347890
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4405341.8093313219338512TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC858850.051.9900178
GAGCACA863600.051.8584759
ATCGGAA881600.050.4534072
TCGGAAG882200.050.445913
CGGAAGA888350.050.1982844
GATCGGA899650.049.3624341
AAGAGCA960250.046.5895087
TCGTATG491900.045.05105244-45
CGTATGC493800.044.95827546-47
TATGCCG497750.044.5486948-49
CTCGTAT500150.044.08947844-45
TATCTCG504950.043.59546340-41
GAAGAGC1031900.043.4585466
TCTCGTA510650.043.09085542-43
ATCTCGT525500.041.9680842-43
ATGCCGT538350.041.1184348-49
ACCGCTC545950.040.63809232-33
GCCGTCT547450.040.42881850-51
GTATGCC549250.040.4281246-47
CGCTCAT553400.040.1507834-35
Produced by FastQC (version 0.11.5)