FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25704623
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4007691.559131989603582TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC825450.048.9903038
GAGCACA831450.048.8712779
ATCGGAA835850.048.0826842
TCGGAAG839650.047.9554183
CGGAAGA854350.047.224824
GATCGGA856800.046.7851941
TCGTATG448550.044.69759444-45
CGTATGC452800.044.3232446-47
TATGCCG456000.044.01164648-49
CTCGTAT455950.043.8679744-45
AAGAGCA930550.043.5235027
TATCTCG460950.043.1607140-41
TCTCGTA468650.042.4926142-43
ATCTCGT485700.041.0889842-43
GAAGAGC1004500.040.3785976
ATGCCGT501300.040.05348248-49
GTATGCC508950.039.4659446-47
ACCGCTC513800.039.1546632-33
CGCTCAT514200.039.07758734-35
GCCGTCT514250.038.974250-51
Produced by FastQC (version 0.11.5)