FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23341786
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4788852.0516210713267613TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA903600.053.7045829
AGAGCAC902750.053.665478
ATCGGAA923900.052.1993642
TCGGAAG926050.052.1496623
CGGAAGA933700.051.813974
GATCGGA946350.050.8256261
AAGAGCA998700.048.6051037
GAAGAGC1076000.045.208566
TCGTATG530600.045.1949344-45
CGTATGC533400.045.11195446-47
TATGCCG538750.044.62381748-49
CTCGTAT537300.044.4854844-45
TATCTCG539400.044.12852540-41
TCTCGTA546950.043.5109342-43
ATCTCGT561550.042.53194442-43
ATGCCGT575000.041.74035348-49
ACCGCTC587750.040.91068332-33
GCCGTCT585900.040.8731950-51
GTATGCC588850.040.83164646-47
CGCTCAT590200.040.7926634-35
Produced by FastQC (version 0.11.5)