FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32305320
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6620942.049489062482588TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1191100.055.2722178
GAGCACA1198900.054.9803929
ATCGGAA1212500.054.0373542
TCGGAAG1215750.053.939293
CGGAAGA1234250.053.1424334
GATCGGA1239650.052.7583051
AAGAGCA1320950.049.886217
GAAGAGC1421350.046.4244166
TCGTATG719200.045.17287444-45
CGTATGC725550.044.8810646-47
TATGCCG728450.044.63665848-49
CTCGTAT729300.044.3714144-45
TATCTCG734700.043.85518640-41
TCTCGTA742700.043.4180142-43
ATCTCGT765800.042.2230942-43
ATGCCGT783700.041.44133448-49
ACCGCTC796600.040.90728432-33
GTATGCC800450.040.68440646-47
CGCTCAT804900.040.51180334-35
GCCGTCT802400.040.45350350-51
Produced by FastQC (version 0.11.5)