FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21450852
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT849070.3958211077117123TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT456140.2126442343642108TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT446630.2082108440261487TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT310850.1449126589470665TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTAT262040.12215831800060901TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC728200.048.1359868
GAGCACA737400.047.70399
TCGGAAG780000.044.842333
ATCGGAA782000.044.6487772
CGGAAGA786950.044.4644134
GATCGGA809550.043.094221
AAGAGCA823700.042.5958487
AATCGGG290250.041.81825640-41
GAATCGG300650.040.9326340-41
AATCGCG57600.040.7016440-41
AGAATCG369650.040.15602538-39
CACTCCG438000.039.7605530-31
GAAGAGC891000.039.469086
ACTCCGG452200.038.5905232-33
CGGAGAA466400.037.3781136-37
TCCGGAG473700.036.91417734-35
CTCCGGA476550.036.68847332-33
CCGGAGA477750.036.59130534-35
GTCACTC482800.036.16394428-29
ATCGCGT28700.035.50554742-43
Produced by FastQC (version 0.11.5)