FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n01_HFFLm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n01_HFFLm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22096029
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT732800.33164330115605845TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT387520.17537992912663175TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT384860.17417609290791572TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT259210.11731067152382901TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC658800.046.4439628
GAGCACA665100.046.1258479
TCGGAAG670950.045.5685963
ATCGGAA671350.045.3930552
CGGAAGA685200.044.6001134
GATCGGA693200.043.9074171
AATCGCG47400.040.9913440-41
AATCGGG259850.040.84205640-41
AAGAGCA751350.040.7358977
GAATCGG270550.039.56918740-41
AGAATCG326300.039.2229238-39
CACTCCG388200.039.1278430-31
GAAGAGC823300.037.3497966
ACTCCGG406450.037.29976732-33
CGGAGAA423450.035.81223736-37
TCCGGAG424800.035.71094534-35
CTCCGGA430500.035.38706632-33
CCGGAGA430750.035.35553734-35
GTCACTC441350.034.59255228-29
ATCGCGT25550.033.93267442-43
Produced by FastQC (version 0.11.5)