FastQCFastQC Report
Wed 1 Jun 2016
HYGNVBGXX_n02_grads_set03_rna_digest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGNVBGXX_n02_grads_set03_rna_digest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106996822
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2151880.2011162537145262No Hit
TTGAGTGCTATATGACCCATGCATCATGTTTTCTTTGCTATAAATCAGTC2134990.19953770215717248No Hit
TTGAGTCATGATGGCATATGGAGTTTTTCTAGACCCGGGGTTACCATTAG2007500.18762239499038577No Hit
TTGAGTTATGGTCTAGAAAAACTCCATATGCCATCATGACTCATATTTAG1267320.11844463941181355No Hit
TTGAGTTAGCTATCTCCCTAATGAGTGGTTCACCGTTTCATACTAATAAA1184150.11067151134638374No Hit
TTGAGTCTATATTATTCAAGCCTTTTTAAATTTTTTTCTATTCATTAACT1147820.10727608339619657No Hit
TTGAGTACACATTTAATTTAGACTCAGGACCTAGATTCTAGATATACTAG1136330.10620221972574102No Hit
TTGAGATTATGGTTAGTCATCAAGGTGGATGGTTAGAGTTATTGAAGGCC1073600.10033942877294058No Hit
TCAAGAAGTAAAAGACACTTGAAACCCTAATTGTTGGCCAAAATATATTT1073030.10028615616265686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA22689750.0138.717561
TTGAGTT25957450.0134.694691
TTGAGTG10425150.0133.871371
TTGAGTC11843200.0129.231831
TGAGTAC6075200.0104.63612
TGAGTAT12471200.0103.007032
TGAGTTC9546300.0102.153532
GAGTATC4580850.0101.576473
TGAGTTA11876000.0100.5967252
GAGTACT3040700.098.2147143
TGAGTAG8772900.098.101022
TGAGTGC4121000.095.729422
GAGTTAC3713100.095.443123
GAGTACG1337650.095.427423
GAGTTCG2334900.094.6941153
TGAGTCC8331050.093.684132
GAGTAGC3486700.093.21983
TAGTTCG1296050.093.097666
TGAGTCT4416850.091.657222
GAGTTCC3322450.090.250313
Produced by FastQC (version 0.11.2)