FastQCFastQC Report
Wed 1 Jun 2016
HYGNVBGXX_n02_grads_set03_dna_digest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGNVBGXX_n02_grads_set03_dna_digest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104072661
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTCCTCCTATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTG2186380.2100820694879705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1838660.17667079733840954No Hit
TTGAGTCCGTTAGAAGATTCTATTCAATTACATTCCATGACGATTCCGTT1659260.1594328408687465No Hit
TTGAGTTAGCTATGGCGGAGGGTGAGCACATATTGCGAAGGAGTTGGCGG1340390.12879367041455778No Hit
TTGAGTATAAGCTTGGGGAATGGGTTGGAAAAGAAGGAGACTAGATCAGA1165440.11198329982165056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA20539050.0138.612961
TTGAGTT22342350.0135.941011
TTGAGTC10370950.0131.10841
TTGAGTG9931150.0127.131161
TGAGTAT10668950.0102.3633352
GAGTATC6010100.0101.812973
TGAGTTC10133750.099.737692
TGAGTTA7245200.098.67112
TGAGTAC5972600.098.090452
TGAGTAG9572350.096.5978242
CGCCTTA1097600.096.094953
TGAGTCT4658900.094.3320852
TGAGTCC6872800.093.956242
GAGTTCC4038250.093.71783
AGTATCG1686250.092.6390844
TGAGTTT7887200.092.451082
GAGTTAC2707700.090.688413
TGAGTGC4227300.090.3677442
GAGTACT2474100.088.813743
GAGTAGC3763250.088.4895863
Produced by FastQC (version 0.11.2)