FastQCFastQC Report
Wed 1 Jun 2016
HYGNVBGXX_n01_grads_set03_rna_digest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGNVBGXX_n01_grads_set03_rna_digest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106996822
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAAGTAAAAGACACTTGAAACCCTAATTGTTGGCCAAAATATATTT1675000.15654670565822973No Hit
TCGAGCAACCATTATCCACGAGCTCAAACATCCATGACACTGTATATCAC1357190.1268439542998763No Hit
TTGAGTGCTATATGACCCATGCATCATGTTTTCTTTGCTATAAATCAGTC1095320.10236939560690879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA16326700.0134.856541
TTGAGTT19476350.0129.09751
TTGAGTG7583600.0128.246491
TTGAGTC8960200.0122.4875641
TGAGTAC5137650.094.774922
TGAGTAT9979600.094.25182
TGAGTTC7885100.093.316222
GAGTATC3747700.091.873063
TGAGTTA10034800.089.910192
GCCGCCT1029200.089.503941
CCGCCTT1034950.089.088912
GAGTACT2589300.088.480253
TGAGTAG7311550.087.215612
GTGAGTA1523400.087.1040951
TGAGTGC3450900.085.1118852
GAGTTAC3151200.085.048043
GAGTACG1202900.083.812123
GAGTTCG1988800.083.607913
TAGTTCG1146650.082.765866
TGAGTCT3764750.082.663942
Produced by FastQC (version 0.11.2)