FastQCFastQC Report
Wed 1 Jun 2016
HYGNVBGXX_n01_grads_set03_dna_digest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGNVBGXX_n01_grads_set03_dna_digest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104072661
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTCCTCCTATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTG1098800.10558008120883929No Hit
CAAGCACAAAGCACATGCGCTCAATCCAAGTCCATCTCCTCTTCCGTCCC1056670.1015319479531709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA14178000.0133.80011
TTGAGTT15845450.0130.571261
TTGAGTC7513500.0123.456791
TTGAGTG7344800.0117.1670761
CGCCTTA1510450.0104.5151443
GCCGCCT1578300.099.642661
GCCTTAC1834250.094.396264
CCGCCTT1710000.092.3050162
TACACTG1958200.091.6681758
TGAGTAT8430350.090.482532
GAGTATC4786600.090.1448443
TGAGTTC8088100.089.518562
TTACACT2062800.087.391877
TGAGTAC4815100.086.7431262
GAGTTCC3213200.085.731173
TGAGTTA5805500.085.228172
TGAGTCT3786450.083.2322
TGAGTAG7799900.082.064722
AGTATCG1354000.081.8689044
ACACTGA2388250.081.522899
Produced by FastQC (version 0.11.2)