FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38590525
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24815046.4303452725766235No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN496300.12860669814676012No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN494440.12812471455104588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG362650.034.2036173
CGGAAGA374900.033.510354
ATCGGAA377150.033.450192
GATCGGA367750.033.258911
GAGCGTC445900.026.3090659
TCGTGTA231200.025.26604314-15
AGAGCGT475150.025.025788
AAGAGCG515850.023.8867457
GTAGGGA222450.022.35821318-19
AGCGTCG265350.022.16749810-11
TAGGGAA226350.021.62637118-19
CGTGTAT41750.019.76027114-15
TGTAGGG240600.019.62517416-17
GTGTAGG248600.019.32836516-17
GAAGAGC665550.019.2953536
GGAAGAG691550.019.0257915
AGGGAAA331100.016.78208420-21
ATTACGA41050.015.9799354-55
GGGGGAA340800.014.67956220-21
CGTGTAA28050.014.578588514-15
Produced by FastQC (version 0.11.5)