FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33077603
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG179150.014.4635850-51
AAAAGGG520200.013.47819480-81
AAGGGGG510550.013.26234882-83
GGGAAAA553950.012.56890722-23
TATAGGG96800.011.87313148-49
AAAGGGG503500.011.80863980-81
GGGGGAA599700.011.353620-21
AAAAAGG662700.010.60893178-79
TAAGGGG151850.010.54175750-51
AAAAAAG978850.010.0185878-79
CGGGGAT91550.09.4378781
GGGGAAT240400.09.37035662-63
CGGGATT58550.09.243561
GGGGAAA558850.09.13581622-23
ATAGGGG123350.09.12503248-49
CGGGAAT73150.09.0860471
TAAAAAA1026450.08.92333870-71
AGGGGGG745400.08.68888782-83
TATAAGG113600.08.65400748-49
CCGGGAT98400.08.2984071
Produced by FastQC (version 0.11.5)