FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32000199
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382460.11951800674739553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG357400.021.19830750-51
AAAGGGG923750.019.00845380-81
TATAGGG146950.018.93835348-49
GGGAAAA762300.018.26665522-23
AAAAAGG1130600.017.54912278-79
AAAAGGG918500.017.2772380-81
GGGGAAT342500.016.16552262-63
GGGGGAA1048550.015.85983420-21
AAGGGGG1037050.015.58569882-83
ATAGGGG229750.014.84168148-49
GGGAATA253100.013.82894464-65
TAAGGGG228750.013.50769450-51
GGGGAAA888250.013.48437522-23
TAAAAAA1829400.012.94760870-71
AAAAAAG1660100.012.81071378-79
TAGGGGT148950.012.15526238-39
TATAAGG123250.011.63681748-49
GGGGGAT367750.011.430613562-63
GAATAAA398100.011.2018366-67
AGGGGGG1828550.011.15692182-83
Produced by FastQC (version 0.11.5)