FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32446899
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG702200.017.7252280-81
TAGGGGG291200.016.89069250-51
AAAAAGG923400.015.25878378-79
GGGAAAA612450.014.52461322-23
AAAAGGG699250.014.41582780-81
GGGGGAA805350.013.82624920-21
AAGGGGG785250.013.34250282-83
GGGGAAT292600.012.57142562-63
TATAGGG117250.011.66519348-49
GGGGAAA704100.011.4063522-23
TAAAAAA1527750.011.16706170-71
GGGAATA212150.010.75215764-65
AGGGGGG1436750.010.45674682-83
GGGGGAT317800.010.24364762-63
AAAAAAG1374400.09.71306378-79
ATAGGGG180650.09.464046548-49
GGGGGTT468650.09.39931940-41
GGGGGGA1357700.09.35971920-21
GGGGTTG391400.09.13589240-41
CGGGAAT82500.09.0346851
Produced by FastQC (version 0.11.5)