FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35733306
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG243450.018.1651550-51
AAAAGGG676250.015.82921980-81
AAGGGGG703400.015.30310282-83
GGGAAAA679550.015.14856122-23
AAAGGGG668450.015.12268680-81
TATAGGG132850.015.06797148-49
GGGGGAA787050.013.67341320-21
GGGGAAT292300.013.0962762-63
AAAAAGG899600.013.00644978-79
TAAGGGG195600.012.55511850-51
GGGAATA217850.011.277925564-65
TATAAGG133650.011.15782348-49
AAAAAAG1337550.011.04871578-79
GGGGAAA741000.010.75813122-23
ATAGGGG178750.010.6009748-49
TTATAGG155350.010.39323746-47
TTTAGGG201200.010.26786348-49
AGGGGGG1100600.010.18152382-83
TTAGGGG195700.010.03467848-49
TAAAAAA1496400.09.75882270-71
Produced by FastQC (version 0.11.5)