FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31953680
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG289350.018.55815150-51
AAAGGGG741650.017.03943680-81
GGGAAAA675400.016.52830322-23
AAAAGGG738400.015.94432480-81
AAAAAGG959550.015.08230778-79
AAGGGGG806150.014.79348882-83
TATAGGG124750.014.78984448-49
GGGGGAA844200.014.49845920-21
GGGGAAT291050.014.08312162-63
ATAGGGG183050.012.27169348-49
GGGGAAA762350.012.00795622-23
GGGAATA218800.011.84798964-65
AAAAAAG1419550.011.32492578-79
TAAAAAA1551400.011.1622270-71
TAAGGGG188850.011.05425650-51
AGGGGGG1400150.010.2420282-83
GAATAAA357200.09.73088766-67
GGGGGAT308650.09.61553862-63
ATAAAAA1094550.09.26719268-69
TATAAGG119150.09.22720248-49
Produced by FastQC (version 0.11.5)