FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35325936
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG722750.015.35307880-81
TAGGGGG255350.015.14204150-51
AAAAGGG713800.014.57460780-81
GGGAAAA644850.014.48709322-23
AAAAAGG930750.013.67246978-79
AAGGGGG797850.013.31926582-83
GGGGGAA807750.012.935060520-21
GGGGAAT300650.011.36516262-63
GGGGAAA728550.010.66822622-23
TAAAAAA1513300.010.27935370-71
AAAAAAG1389950.010.25829578-79
GGGAATA227250.09.57751764-65
AGGGGGG1367000.09.38551982-83
ATAGGGG178450.09.31470848-49
TATAGGG117600.09.16719148-49
CTCGGAT90650.08.7983851
CGCGATT13007.2759576E-128.76454451
TAAGGGG189550.08.65805150-51
CGCGGGT74000.08.5967551
GGGGGGA1299050.08.4947420-21
Produced by FastQC (version 0.11.5)