FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30684551
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322240.10501701654360202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG322300.020.16906450-51
AAAGGGG751750.019.05735480-81
GGGAAAA697500.018.44582222-23
AAAAGGG769850.018.07592880-81
AAGGGGG827000.016.98927782-83
AAAAAGG991750.016.56573578-79
GGGGGAA926200.015.7565620-21
TATAGGG126450.015.64296548-49
GGGGAAT307200.015.23804362-63
GGGGAAA803950.013.14715522-23
GGGAATA224750.013.10944364-65
AAAAAAG1466750.012.76370478-79
TAAAAAA1616100.012.72191870-71
TTAGGGG206800.011.85010148-49
TAAGGGG191000.011.47729550-51
AGGGGGG1460000.011.38609282-83
ATAGGGG191500.011.22206348-49
TTTAGGG197500.011.16970248-49
GGGGGAT319900.011.03795862-63
AAATGGG368400.010.53379780-81
Produced by FastQC (version 0.11.5)