FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38216086
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404380.10581408048956138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG359000.019.2123350-51
AAAGGGG849650.018.43120880-81
AAAAAGG1092900.015.54471778-79
AAAAGGG845800.013.61422378-79
TATAGGG138700.013.439157548-49
GGGGGAA975600.013.38592720-21
GGGAAAA660900.012.04387322-23
AGGGGGG1779500.011.98190782-83
AAGGGGG967350.011.85072880-81
TAAAAAA1797100.011.6850470-71
AAAAAAG1621550.011.58346576-77
GGGGAAA774150.011.17766822-23
GGGGAAT356150.010.96207562-63
TTAGGGG227150.010.80902848-49
GGGGGAT473300.010.27707362-63
GGGGGGA1887550.09.96830320-21
CGGGGAT121400.09.85471251
TTTAGGG226250.09.750030548-49
ATAGGGG222950.09.55352948-49
GTAAAAA486750.09.50474968-69
Produced by FastQC (version 0.11.5)