FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31835633
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG316000.022.13439450-51
TATAGGG142750.019.1464748-49
AAAGGGG801650.018.8279880-81
GGGAAAA735150.017.82718822-23
AAAAGGG804100.017.65778280-81
AAAAAGG1016400.016.88763878-79
AAGGGGG890450.016.16003882-83
GGGGGAA942600.015.60863820-21
TAAGGGG221350.015.23625750-51
GGGGAAT331850.014.87928962-63
TATAAGG130650.014.06763648-49
ATAGGGG205250.013.69802948-49
GGGAATA232650.013.17499464-65
GGGGAAA821550.013.17174822-23
TTTAGGG206150.012.86646848-49
TTATAGG150350.012.85510646-47
TTAGGGG209800.012.84635548-49
AAAAAAG1509500.012.67178-79
TAAAAAA1670050.012.54275770-71
GTTTGGG322350.012.42397130-31
Produced by FastQC (version 0.11.5)