FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31125254
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG226200.017.4610950-51
AAAGGGG590150.014.94102780-81
AAAAGGG602350.014.32712380-81
GGGAAAA558850.014.022387522-23
AAGGGGG636600.013.63852382-83
GGGGGAA675550.013.09616320-21
TATAGGG112350.012.95604648-49
GGGGAAT251500.012.9057962-63
AAAAAGG789550.012.89535978-79
ATAGGGG161550.011.36207548-49
GGGGAAA619900.010.73370822-23
GGGAATA196300.010.59118764-65
AAAAAAG1167250.010.25944778-79
TAAGGGG167900.010.241405550-51
TAAAAAA1287100.09.84796570-71
AGGGGGG1043150.09.60449982-83
CGCGAGT27650.09.10014251
GGGGGGA1021250.08.88339460-61
CGCGGGT62650.08.5629771
GGGGGAT256200.08.51403562-63
Produced by FastQC (version 0.11.5)