FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27435341
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG214750.016.92049850-51
GGGAAAA535750.015.42076522-23
AAAAGGG544050.015.39145480-81
AAAGGGG537600.014.80790780-81
AAGGGGG585100.014.0477482-83
GGGGGAA626900.013.8132820-21
AAAAAGG718400.012.88781978-79
TATAGGG94000.012.78224248-49
GGGGAAT232550.012.35393762-63
GGGGAAA586950.011.16257222-23
AAAAAAG1061800.010.95888178-79
CGCGATT9851.8189894E-1210.6035071
ATAGGGG136350.010.53621448-49
GGGAATA178900.010.49288964-65
TAAAAAA1168400.010.02745770-71
TAAGGGG147000.09.61290250-51
GGGGGTT321600.09.48599740-41
GGGGTTG291150.09.20505340-41
GGGGGGA954850.09.01142860-61
AGGGGGG1013400.08.766623582-83
Produced by FastQC (version 0.11.5)