FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29682268
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG201700.018.87541850-51
AAAAGGG621450.017.25957980-81
GGGAAAA625700.017.13172122-23
AAGGGGG646350.016.36015382-83
AAAGGGG594100.015.07374980-81
TATAGGG118300.014.97418648-49
GGGGGAA728050.014.30844220-21
TAAGGGG177050.014.00469150-51
GGGGAAT263050.013.6854662-63
TATAAGG118950.012.83617648-49
AAAAAGG813350.012.7809478-79
AAAAAAG1209250.012.31498478-79
GGGGAAA676000.011.67318222-23
GGGAATA194900.011.54530164-65
TTTAGGG175200.010.748013548-49
TTATAGG133500.010.65365146-47
ATAGGGG155150.010.637073548-49
ATAAGGG155650.010.37406448-49
TAAAAAA1353650.010.11280670-71
TTATAAG141250.010.06911446-47
Produced by FastQC (version 0.11.5)