FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n02_HFFLm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n02_HFFLm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28737010
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG244150.018.01564650-51
GGGAAAA596000.016.7183722-23
AAAAGGG639650.016.54940680-81
AAAGGGG642250.015.99828480-81
AAGGGGG709200.014.946877582-83
AAAAAGG830900.014.34405978-79
GGGGGAA739950.014.26794420-21
GGGGAAT264600.013.56958362-63
TATAGGG114350.013.04096448-49
GGGGAAA670600.011.82027322-23
GGGAATA193150.011.66244664-65
AAAAAAG1252250.011.65310878-79
ATAGGGG162750.011.55554448-49
TAAGGGG171350.011.26865550-51
TAAAAAA1373300.010.64902870-71
AGGGGGG1198550.09.78715382-83
GGGGGAT270300.09.46807262-63
TATAAGG102400.09.41482548-49
GAATAAA326000.09.27751366-67
ATAAAAA970600.09.23216268-69
Produced by FastQC (version 0.11.5)