FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38590525
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29258997.581910326433755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1143000.2961866934953593TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC933950.065.2469258
CGGAAGA977250.063.4967084
TCGGAAG972000.063.4960333
ATCGGAA994400.062.448452
GATCGGA970550.061.8917161
GAGCACA1126350.054.161799
AAGAGCA1206350.051.7007877
GAAGAGC1232300.050.116216
GGAAGAG1323200.047.2613265
TCTCGTA370150.041.45578842-43
ATGCCGT436000.039.8091948-49
CTCGTAT376000.038.5294144-45
ATCTCGT377450.036.6435742-43
ACGTCTG840400.035.9956714-15
ACACGTC842800.035.9109712-13
CGTCTGA838250.035.8221716-17
CACGTCT839750.035.69655214-15
CACACGT847250.035.53399312-13
GCTTGAA477800.035.4965960-61
GCACACG865000.034.8620510-11
Produced by FastQC (version 0.11.5)