FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33077603
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3857521.1662030044921936TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1009800.044.256629
TCGTATG489450.043.99408744-45
CGTATGC492600.043.88258746-47
AGAGCAC1024550.043.554198
TATGCCG500000.043.18976248-49
TCGTATC473600.043.0522538-39
CTCGTAT482750.043.02078644-45
CGTATCT477350.042.3955438-39
ATTCGTA516350.042.05792636-37
CGGAAGA1063900.041.8594174
TATCTCG486700.041.68842340-41
ATCGGAA1068500.041.6224332
TTCGTAT510250.041.5643436-37
TCGGAAG1071850.041.5583043
AATTCGT531000.041.06861534-35
TCTCGTA498100.040.8746842-43
ACGAATT542600.040.6970632-33
GATCGGA1088900.040.6365781
GTCACGA548650.040.2495328-29
GAATTCG545500.040.20343834-35
Produced by FastQC (version 0.11.5)