FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32000199
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT10455413.2672953065073127TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT332100.10378060461436506TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1831250.065.363529
AGAGCAC1849900.064.7426458
CGGAAGA1924700.062.2225844
TCGGAAG1935100.061.9010243
ATCGGAA1958200.061.1256832
AAGAGCA1982050.060.5242657
GATCGGA1989650.059.8032531
GAAGAGC2075800.057.9015056
GGAAGAG2287000.052.641625
TCGTATG1247100.046.05193344-45
CGTATGC1256400.045.73647346-47
TATGCCG1257250.045.65587648-49
CTCGTAT1192600.045.62167744-45
TCTCGTA1196250.045.24879542-43
ATCTCGT1201000.044.58140642-43
AATCTCG1204950.044.5655440-41
ATGCCGT1301450.044.07976548-49
GCCGTCT1292100.043.8052250-51
GTATGCC1329850.043.38355646-47
CCGTCTT1307750.043.1700152-53
Produced by FastQC (version 0.11.5)