FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32446899
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT7493592.3094934280160335TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1444900.058.7093669
AGAGCAC1456200.058.2858628
CGGAAGA1527400.055.607194
TCGGAAG1532350.055.520713
ATCGGAA1549200.054.801112
AAGAGCA1593050.053.4338577
GATCGGA1583250.053.2572671
GAAGAGC1687200.050.5431186
TCGTATG896750.045.67911544-45
CGTATGC904050.045.38553246-47
TATGCCG907600.045.13681848-49
CTCGTAT862050.044.9807944-45
GGAAGAG1915850.044.6671375
TCTCGTA865550.044.48192242-43
AATCTCG877050.043.6616340-41
ATCTCGT879350.043.4030542-43
ATGCCGT958950.042.7396348-49
GCCGTCT951150.042.50347550-51
GTATGCC971100.042.40102446-47
CCGTCTT966700.041.7412452-53
Produced by FastQC (version 0.11.5)