FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35733306
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT6312441.766542396049221TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1337250.053.519799
AGAGCAC1343400.053.2955448
CGGAAGA1398050.051.2502174
TCGGAAG1400850.051.114023
ATCGGAA1409850.050.724512
GATCGGA1434250.049.480991
AAGAGCA1482200.048.4713027
TCGTATG764050.045.1188544-45
GAAGAGC1605500.044.8203356
CGTATGC773100.044.72856546-47
TATGCCG775750.044.59311348-49
CTCGTAT737850.044.3940844-45
TCTCGTA751800.043.25876242-43
AATCTCG757050.042.745440-41
ATCTCGT762450.042.35549542-43
ATGCCGT832950.041.5707448-49
GCCGTCT835050.040.92878350-51
GTATGCC855950.040.62663346-47
CCGTCTT850250.040.1661552-53
TGCCGTC872700.039.459650-51
Produced by FastQC (version 0.11.5)