FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31953680
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT7615772.3833780647487237TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1422550.061.2118159
AGAGCAC1434950.060.722198
TCGGAAG1480250.058.8713463
CGGAAGA1478850.058.8528564
ATCGGAA1496850.058.1239282
GATCGGA1517500.056.948641
AAGAGCA1556950.056.040357
GAAGAGC1649550.053.0044176
GGAAGAG1852250.047.321845
TCGTATG918850.045.73874344-45
CGTATGC927800.045.4873546-47
TATGCCG927100.045.46179248-49
CTCGTAT883000.045.18073344-45
TCTCGTA894200.044.2506242-43
ATCTCGT895750.043.8214242-43
AATCTCG895600.043.7726540-41
ATGCCGT974500.043.0945848-49
GCCGTCT970200.042.68112650-51
GTATGCC994400.042.5339646-47
CCGTCTT986400.042.0426152-53
Produced by FastQC (version 0.11.5)