FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35325936
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT6784721.9206058687305554TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1413750.055.2865149
AGAGCAC1420450.054.9951068
TCGGAAG1484350.052.6641463
CGGAAGA1486750.052.434954
ATCGGAA1498850.052.0859262
GATCGGA1528200.050.790351
AAGAGCA1568650.049.8871777
GAAGAGC1680900.046.6808246
TCGTATG829600.045.47242744-45
CGTATGC842550.044.87699546-47
TATGCCG842050.044.80992548-49
CTCGTAT796900.044.56706244-45
TCTCGTA808450.043.66128542-43
AATCTCG821750.042.61831740-41
ATCTCGT820600.042.53441242-43
ATGCCGT896300.042.03415348-49
GCCGTCT892350.041.55230750-51
GTATGCC920650.041.16029446-47
GGAAGAG1933700.040.732785
CCGTCTT913700.040.5525352-53
Produced by FastQC (version 0.11.5)