FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30684551
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT8971212.9236895139837635TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1617500.062.194339
AGAGCAC1625100.061.9145478
CGGAAGA1691900.059.378084
TCGGAAG1706300.058.9132423
ATCGGAA1725850.058.1476822
AAGAGCA1760350.057.2250147
GATCGGA1751900.056.8905141
GAAGAGC1850550.054.5056426
GGAAGAG2048350.049.3744245
TCGTATG1060750.045.83232544-45
TATGCCG1068650.045.62666748-49
CGTATGC1071400.045.61878246-47
CTCGTAT1018000.045.41266644-45
TCTCGTA1023900.044.7909942-43
ATCTCGT1030950.044.10920742-43
AATCTCG1035350.043.9716440-41
ATGCCGT1112050.043.66237348-49
GCCGTCT1106050.043.2357750-51
GTATGCC1133650.043.09914446-47
CCGTCTT1122250.042.645552-53
Produced by FastQC (version 0.11.5)