FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38216086
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT9920552.5959094816774275TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1870050.061.0484779
AGAGCAC1874750.060.9809958
CGGAAGA1954200.058.509884
TCGGAAG1957000.058.4653783
ATCGGAA1977000.057.7955632
GATCGGA2006000.056.627231
AAGAGCA2036000.056.2353067
GAAGAGC2154150.053.2839626
GGAAGAG2410050.047.7050635
TCGTATG1197250.045.8208844-45
CGTATGC1210550.045.51286746-47
TATGCCG1212250.045.36998448-49
CTCGTAT1149250.045.2737444-45
TCTCGTA1162450.044.50031742-43
AATCTCG1170100.043.89030540-41
ATCTCGT1171950.043.77888542-43
ATGCCGT1271300.043.2009848-49
GCCGTCT1256300.043.03245550-51
GTATGCC1289950.042.7482446-47
CCGTCTT1276250.042.36903452-53
Produced by FastQC (version 0.11.5)