FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31835633
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT9044522.841005234606141TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1682350.061.10199
AGAGCAC1701500.060.4447178
CGGAAGA1766300.058.2362674
TCGGAAG1770050.058.161453
ATCGGAA1791900.057.38682
GATCGGA1820300.056.1481131
AAGAGCA1836550.056.108547
GAAGAGC1928650.053.5211756
GGAAGAG2136250.048.404485
TCGTATG1082250.045.79225544-45
CGTATGC1090400.045.61998746-47
TATGCCG1095100.045.44274548-49
CTCGTAT1042950.045.32973544-45
TCTCGTA1047950.044.76701442-43
AATCTCG1057050.043.98083540-41
ATCTCGT1059050.043.96591642-43
ATGCCGT1142150.043.4501948-49
GCCGTCT1130850.043.26060550-51
GTATGCC1159850.042.9947946-47
CCGTCTT1147100.042.68699652-53
Produced by FastQC (version 0.11.5)