FastQCFastQC Report
Wed 6 Mar 2019
HYGKFBGX9_n01_HFFLm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGKFBGX9_n01_HFFLm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31125254
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT5299881.7027587951571417TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1146500.054.694289
AGAGCAC1158800.054.1420678
TCGGAAG1213150.051.568713
CGGAAGA1217400.051.4156574
ATCGGAA1226150.050.9528272
GATCGGA1252450.049.535161
AAGAGCA1287050.048.7691357
GAAGAGC1378950.045.6193776
TCGTATG667850.045.36311344-45
CGTATGC674350.045.28111646-47
TATGCCG675850.044.99361448-49
CTCGTAT646400.044.78547344-45
TCTCGTA634600.043.56099742-43
ATCTCGT642250.042.5429342-43
GTCACGA732350.042.31478528-29
CGAGATT736200.041.75705732-33
ATGCCGT726850.041.64060648-49
AGTCACG749200.041.6008528-29
ACGAGAT743700.041.52442632-33
GCCGTCT724200.041.4027550-51
Produced by FastQC (version 0.11.5)